Journal: PLoS ONE

Article Title: Mesenchymal Transition and PDGFRA Amplification/Mutation Are Key Distinct Oncogenic Events in Pediatric Diffuse Intrinsic Pontine Gliomas

doi: 10.1371/journal.pone.0030313

Figure Lengend Snippet: Panel A: Radial plot showing the expression of oligodendroglial markers in the two groups of DIPG, in log2 ratios related each other. The red circle represent the median expression level of the whole population of DIPG. Group 1 expresses higher levels of oligodendroglial markers than group 2 DIPG. Panel B: Morphological oligodendroglial differenciation in group 1 tumors (HES staining, ×40). Panel C: Morphological astrocytic differenciation in group 2 tumors (HES staining ×40). Panel D: Olig2 immunohistochemistry in a group 1 DIPG showing that probably not all cells in the biopsy are tumoral (×40). Panel E: Dual immunohistochemistry for Olig2 and GFAP showing that tumor cells in mitosis are GFAP negative but Olig2 positive (×100). Panel F: Overall survival of 55 DIPG according to the presence (red) or absence (blue) of oligodendroglial differenciation. Median OS was shorter in patients with oligodendroglial type of tumors (7.73 vs 12.37, p = 0.045, log rank test).

Article Snippet: Sections were then incubated with various commercial monoclonal primary antibodies against Olig2 (AF 2418, 1/150, R/D system, CA, USA), P53 (DO-1, 1/1, Ventana) and MIB-1 (1/100; Dako, Glostrup, Denmark).

Techniques: Expressing, Staining, Immunohistochemistry

Journal: Science Advances

Article Title: Oriented cell divisions induce basal progenitors and regulate neural expansion across tissues and species

doi: 10.1126/sciadv.adz6827

Figure Lengend Snippet: ( A ) Representative images of adult cortical sections from dHET and dKO stained for markers of upper layer II/III neurons (BRN2), deeper layers IV/V neurons (CTIP2), and astrocytes (SOX9). Scale bar, 200 μm. ( B ) Quantification of cell type proportions in adult dHET cortex, dKO cortex, and dKO subcortical heterotopia band. Upper layer II/III, deeper layer IV/V neurons, astrocytes, interneurons, and oligodendrocytes cell types are identified using BRN2/Pou3f2, CTIP2, SOX9, GAD67, and OLIG2, respectively. ( C ) Representative images of adult retinal sections from dHET and dKO stained for markers of amacrines (PAX6), bipolars (CHX10), retinal ganglion cells (BRN3a/POU4F1), Müller cells (LHX2), cones (ARR3), rods (NRL), and horizontals (LIM1). Yellow dashed lines underlie the extra retinal layer of dKO retina, white dashed lines underlie the expanded ganglion cell layer of dKO retina. Scale bar, 100 μm. ( D ) Quantification of retinal cell type proportions in adult dHET and dKO retina (excluding the extra layer, gray and black bars) and in the extra layer (red bars). Error bars represent mean ± SEM. All comparisons between groups were done using a one-way ANOVA test followed by Tukey’s correction for multiple comparisons. (* P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001).

Article Snippet: OLIG2 , Rabbit , 1/500 , PhosphoSolutions , AB_2492193.

Techniques: Staining

Journal: Molecular Cancer Research

Article Title: BMP Signaling Induces Astrocytic Differentiation of Clinically Derived Oligodendroglioma Propagating Cells

doi: 10.1158/1541-7786.mcr-13-0349

Figure Lengend Snippet: Figure 3. OligPCs form tumors that resemble oligodendrogliomas in vivo. A, Kaplan–Meier survival curves showing tumorigenicity of 2 OligPC lines following orthotopic transplantation in 6 mice each. B, H&E staining of an OligPC xenograft shows cytoplasmic clearing, spontaneous hemorrhage (arrow), and fine branching vasculature (arrowhead). Dashed line, tumor margin. Magnification, 10. C, immunostaining with a human- specific nestin antibody confirms xenograft origin and demonstrates invading cells (arrowheads) beyond the tumor margin (dashed line). Scale bar, 100 mm. D, immunostaining with lineage markers reveals robust OLIG2 expression with a relative paucity of GFAP expression in xenografts; merged with DAPI nuclear counterstain at far right. Scale bar, 100 mm.

Article Snippet: Primary antibodies used were: GFAP (DakoCytomaton; rabbit polyclonal, 1:1,000), OLIG2 (R&D Systems; goat polyclonal, 1:1000), human-specific Nestin (BD Biosciences; mouse IgG1, 1:500).

Techniques: In Vivo, Transplantation Assay, Staining, Immunostaining, Expressing

Journal: Molecular Cancer Research

Article Title: BMP Signaling Induces Astrocytic Differentiation of Clinically Derived Oligodendroglioma Propagating Cells

doi: 10.1158/1541-7786.mcr-13-0349

Figure Lengend Snippet: Figure 7. OLIG proteins colocalize with ID2 and ID4 in BMP-treated OligPCs. A–D, OligPCs were grown with or without 100 ng/mL BMP4 for 12 hours, followed by immunostaining for OLIG1 þ ID2 (A), OLIG1 þ ID4 (B), OLIG2 þ ID2 (C), and OLIG2 þ ID4 (D), with DAPI nuclear counterstain. Scale bars, 50 mm. Zoomed images of the boxed areas demonstrate nuclear (A0) and cytoplasmic (A00) Olig localization. Scale bars, 25 mm. E, OLIG1 or OLIG2 protein was immunoprecipitated from OligPCs grown as in A–D and Western blot analysis was performed for ID2 and ID4.

Article Snippet: Primary antibodies used were: GFAP (DakoCytomaton; rabbit polyclonal, 1:1,000), OLIG2 (R&D Systems; goat polyclonal, 1:1000), human-specific Nestin (BD Biosciences; mouse IgG1, 1:500).

Techniques: Immunostaining, Immunoprecipitation, Western Blot